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Issue 3, 2015
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The in vitro identification and quantification of volatile biomarkers released by cystic fibrosis pathogens

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Abstract

There is interest in the development of exhaled breath tests for the detection of lower airway infection in children with cystic fibrosis. The first stage of this process is the identification of volatile organic compounds (VOCs) released into the gas phase by CF pathogens that can be used as breath test biomarkers. Selected ion flow tube mass spectrometry (SIFT-MS) is ideally suited to these in vitro studies as it allows simultaneous quantification of multiple VOCs in real time. We review a decade of in vitro experiments using SIFT-MS to analyse the VOCs released by respiratory pathogens. This includes identification and quantification of VOCs and the investigation of the in vitro factors that affect their production. We also report on how our culture methodology has been refined over the years to better account for variations in bacterial mass. Finally, we discuss how these in vitro findings have been translated into clinical trials and assess possible future applications.

Graphical abstract: The in vitro identification and quantification of volatile biomarkers released by cystic fibrosis pathogens

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Publication details

The article was received on 15 Dec 2014, accepted on 06 Jan 2015 and first published on 07 Jan 2015


Article type: Minireview
DOI: 10.1039/C4AY02981J
Citation: Anal. Methods, 2015,7, 818-824
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    The in vitro identification and quantification of volatile biomarkers released by cystic fibrosis pathogens

    F. J. Gilchrist, P. Španěl, D. Smith and W. Lenney, Anal. Methods, 2015, 7, 818
    DOI: 10.1039/C4AY02981J

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