Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 6, 2015
Previous Article Next Article

Detection of sequence-specific DNA with a morpholino-functionalized silicon chip

Author affiliations

Abstract

In this work, an efficient method for the sequence-specific detection of DNA based on a morpholino-functionalized silicon chip platform has been proposed. Briefly, morpholino was first immobilized on the surface of a silicon chip using 3-aminopropyltriethoxysilane (APTES) as the silane coupling agent and 1,4-phenylenediisothiocyanate (PDITC) as the cross-linker and then hybridized with DNA in the ensuing step. The fluorescence label was introduced by strongly binding Rhodamine B, which contains a terminal carboxylic group, with DNA by means of phosphate–zirconium–carboxylate coordination reaction. X-ray photoelectron spectroscopy (XPS) was used to characterize the silicon surface. Under optimal conditions, the morpholino-functionalized silicon chip presented a great linear relationship between the fluorescence intensity and the logarithm of target DNA concentrations in the range from 1 pM to 1 nM with a detection limit of 4.52 pM. Furthermore, fully complementary versus single-base mismatched, three-base mismatched and non-complementary DNA could be effectively identified. The chip showed excellent stability because it could be reused for another hybridization experiment after denaturing the morpholino–complementary DNA duplex. In addition, the chip rendered satisfactory analytical performance for the detection of DNA in serum samples, thus exhibiting practical significance. Morpholino-functionalized silicon chips display good sensitivity and selectivity for the detection of DNA and promising applications in single-nucleotide polymorphisms (SNPs).

Graphical abstract: Detection of sequence-specific DNA with a morpholino-functionalized silicon chip

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 21 Nov 2014, accepted on 27 Jan 2015 and first published on 27 Jan 2015


Article type: Paper
DOI: 10.1039/C4AY02780A
Citation: Anal. Methods, 2015,7, 2406-2412
  •   Request permissions

    Detection of sequence-specific DNA with a morpholino-functionalized silicon chip

    W. Hu, Q. Hu, L. Li, J. Kong and X. Zhang, Anal. Methods, 2015, 7, 2406
    DOI: 10.1039/C4AY02780A

Search articles by author