Issue 4, 2016

Use of Raman optical tweezers for cell cycle analysis

Abstract

We report the results of our investigations on the use of Raman optical tweezers for label free analysis of cells in different phases of their cell cycle. The studies performed on human colon adenocarcinoma (Colo-205) cells synchronized in G0/G1 and G2/M phases showed that the DNA Raman band at 783 cm−1 in the Raman spectra of optically trapped cells can provide information about the DNA content in the nucleus of the cell without the need for the isolation of the nucleus. The histograms of intensity of this band among the cell populations were found to corroborate the results obtained from fluorescence image cytometry performed on DAPI stained cells.

Graphical abstract: Use of Raman optical tweezers for cell cycle analysis

Article information

Article type
Paper
Submitted
14 May 2015
Accepted
16 Dec 2015
First published
21 Dec 2015

Analyst, 2016,141, 1339-1346

Use of Raman optical tweezers for cell cycle analysis

S. Ahlawat, A. Chowdhury, A. Uppal, N. Kumar and P. K. Gupta, Analyst, 2016, 141, 1339 DOI: 10.1039/C5AN00971E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements