Jump to main content
Jump to site search

Issue 13, 2015
Previous Article Next Article

Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy

Author affiliations

Abstract

Fourier Transform Infrared (FTIR) micro-spectroscopy measurements were acquired to study infrared signatures of chemotherapeutic response as a function of the cell cycle. Renal carcinoma Caki-2 cells were exposed to IC50 doses of 5-fluorouracil and Paclitaxel for a period of 24 hours. The inherent cell cycle infrared signatures from untreated and drug-treated cells were successfully retrieved by the construction of a robust SVM able to discriminate the cell cycle phases of this cell line with an average accuracy of 83.7%. The overriding infrared signature observed relates to an apoptotic biochemical response that does not appear to be correlated with the events affected by the drugs’ mode of action or the cell cycle. Since apoptosis is a well conserved mechanism among living species, these results suggest that both the stages of proliferation as well as the absence/presence of apoptosis need to be taken into account in order to elucidate the fine biochemical details revealing the immediate cellular response to the drug in order to assign reliable spectral patterns of drug action.

Graphical abstract: Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Apr 2015, accepted on 18 May 2015 and first published on 01 Jun 2015


Article type: Paper
DOI: 10.1039/C5AN00671F
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 4453-4464
  •   Request permissions

    Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy

    M. Jimenez-Hernandez, M. D. Brown, C. Hughes, N. W. Clarke and P. Gardner, Analyst, 2015, 140, 4453
    DOI: 10.1039/C5AN00671F

Search articles by author

Spotlight

Advertisements