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Issue 16, 2015
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An integrated ceramic, micro-fluidic device for the LC/MS/MS analysis of pharmaceuticals in plasma

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Abstract

An integrated capillary scale (300 μm id) ceramic microfluidic LC system combined with MS/MS has been successfully employed for the quantitative analysis of pharmaceutical compounds in human plasma. The capillary ceramic microfluidic LC/MS/MS system showed an approximate 20-fold (range 11–38-fold) increase in sensitivity compared with a standard 2.1 mm scale UPLC/MS/MS system for a broad range of analytes. The loading capacity of the devices capillary separations channel allowed injection of 2 μL of an aqueous solution, and up to 1.2 μL of a typical protein-precipitated plasma sample, onto the reversed-phase chromatography system. The system also showed excellent chromatographic performance and robustness, with no deleterious effects on the chromatography observed over the course of 1000 injections of protein-precipitated plasma. The ability of the ceramic microfluidic LC/MS/MS system to deliver this level of sensitivity and performance enables the routine quantification of pharmaceutical compounds from small format samples, such as those obtained by dried blood spot or other blood microsampling approaches, to be performed.

Graphical abstract: An integrated ceramic, micro-fluidic device for the LC/MS/MS analysis of pharmaceuticals in plasma

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Publication details

The article was received on 02 Apr 2015, accepted on 12 Jun 2015 and first published on 06 Jul 2015


Article type: Paper
DOI: 10.1039/C5AN00646E
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 5546-5556
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    An integrated ceramic, micro-fluidic device for the LC/MS/MS analysis of pharmaceuticals in plasma

    P. D. Rainville, J. P. Murphy, M. Tomany, I. D. Wilson, N. W. Smith, C. Evans, J. Kheler, C. Bowen, R. S. Plumb and J. K. Nicholson, Analyst, 2015, 140, 5546
    DOI: 10.1039/C5AN00646E

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