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Issue 16, 2015
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Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins

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Abstract

This paper describes a simple microfluidic device that can generate nonlinear concentration gradients. We changed the “width” of channels that can drastically shorten the total microfluidic channel length and simplify the microfluidic network design rather than the “length” of channels. The logarithmic concentration gradients generated by the device were in good agreement with those obtained by simulation. Using this device, we evaluated a probable IC50 value of the ABC transporter proteins by the competitive transport assays at five different logarithmic concentrations. This probable IC50 value was in good agreement with an IC50 value (0.92 μM) obtained at the diluted concentrations of seven points.

Graphical abstract: Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins

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Publication details

The article was received on 30 Nov 2014, accepted on 22 Jun 2015 and first published on 22 Jun 2015


Article type: Paper
DOI: 10.1039/C4AN02201G
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 5557-5562
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    Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins

    Y. Abe, K. Kamiya, T. Osaki, H. Sasaki, R. Kawano, N. Miki and S. Takeuchi, Analyst, 2015, 140, 5557
    DOI: 10.1039/C4AN02201G

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