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Issue 25, 2015
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Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication

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Abstract

4′-SelenoDNA fragments were synthesized for the first time using 4′-selenothymidine triphosphate (SeTTP) by taking advantage of its bioequivalence against DNA polymerases. DNA fragments each with a homologous element (O, S or Se) at the 4′-position of the thymidine units were effectively amplified using KOD Dash DNA polymerase.

Graphical abstract: Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication

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Publication details

The article was received on 09 May 2015, accepted on 20 May 2015 and first published on 20 May 2015


Article type: Communication
DOI: 10.1039/C5OB00941C
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2015,13, 6949-6952
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    Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication

    N. Tarashima, T. Sumitomo, H. Ando, K. Furukawa, T. Ishida and N. Minakawa, Org. Biomol. Chem., 2015, 13, 6949
    DOI: 10.1039/C5OB00941C

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