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Issue 34, 2014
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Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells

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Abstract

The physical and chemical properties of a matrix play an important role in determining various cellular behaviors, including lineage specificity. We demonstrate that the differentiation commitment of human embryonic stem cells (hESCs), both in vitro and in vivo, can be solely achieved through synthetic biomaterials. hESCs cultured using mineralized synthetic matrices mimicking a calcium phosphate (CaP)-rich bone environment differentiated into osteoblasts in the absence of any osteogenic inducing supplements. When implanted in vivo, these hESC-laden mineralized matrices contributed to ectopic bone tissue formation. In contrast, cells within the corresponding non-mineralized matrices underwent either osteogenic or adipogenic fate depending upon the local cues present in the microenvironment. To our knowledge, this is the first demonstration where synthetic matrices are shown to induce terminal cell fate specification of hESCs exclusively by biomaterial-based cues both in vitro and in vivo. Technologies that utilize tissue specific cell–matrix interactions to control stem cell fate could be a powerful tool in regenerative medicine. Such approaches can be used as a tool to advance our basic understanding and assess the translational potential of stem cells.

Graphical abstract: Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells

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Publication details

The article was received on 04 May 2014, accepted on 01 Jul 2014 and first published on 04 Jul 2014


Article type: Paper
DOI: 10.1039/C4TB00714J
Citation: J. Mater. Chem. B, 2014,2, 5676-5688
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    Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells

    H. Kang, C. Wen, Y. Hwang, Y. V. Shih, M. Kar, S. W. Seo and S. Varghese, J. Mater. Chem. B, 2014, 2, 5676
    DOI: 10.1039/C4TB00714J

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