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Issue 32, 2014
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Robust bio-inspired antibacterial surfaces based on the covalent binding of peptides on functional atmospheric plasma thin films

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Abstract

Here, we describe a robust process aiming at conferring antibacterial properties on stainless steel through the covalent grafting of nisin, a natural antimicrobial peptide, onto a functional plasma thin film deposited by an atmospheric pressure dielectric barrier discharge process. The three different steps of the procedure, namely the deposition of a carboxyl rich thin layer, the surface activation by using a zero-length crosslinking agent and the nisin immobilisation, are reported and thoroughly characterised. A correlation between the carboxylic group surface concentration and the surface roughness onto the antibacterial properties of the layers is evidenced. Finally, IR analyses appear as a powerful analytical tool allowing us to validate the different chemical surface modifications, to confirm the relevance of the activation step to achieve a stable and homogenous peptide grafting over all the surfaces, as well as to investigate the secondary structure of immobilized peptides.

Graphical abstract: Robust bio-inspired antibacterial surfaces based on the covalent binding of peptides on functional atmospheric plasma thin films

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Publication details

The article was received on 30 Mar 2014, accepted on 27 May 2014 and first published on 02 Jul 2014


Article type: Paper
DOI: 10.1039/C4TB00503A
Citation: J. Mater. Chem. B, 2014,2, 5168-5177
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    Robust bio-inspired antibacterial surfaces based on the covalent binding of peptides on functional atmospheric plasma thin films

    R. Mauchauffé, M. Moreno-Couranjou, N. D. Boscher, C. Van De Weerdt, A. Duwez and P. Choquet, J. Mater. Chem. B, 2014, 2, 5168
    DOI: 10.1039/C4TB00503A

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