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Issue 24, 2014
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Local and transient permeation events are associated with local melting of giant liposomes

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Abstract

We reveal that the gel to fluid phase transition causes spherical membrane vesicles to release a finite number of molecules in several consecutive and localized events. By locally melting Giant Unilamellar lipid Vesicles (GUVs), using an optically trapped gold nanoparticle (AuNP) as a local heat source, we establish a local phase transition on the spherical GUV membrane clearly visualized using a phase sensitive fluorescent marker. We measure transient permeation events through this transition zone visualized as de-quenching of calcein as it escapes the interior of the GUV. Since biological membranes share several features with melting membranes, like nanoscale domain formation and critical density fluctuations, similar passive membrane transport could potentially be abundant in living cells.

Graphical abstract: Local and transient permeation events are associated with local melting of giant liposomes

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Publication details

The article was received on 20 Feb 2014, accepted on 19 Mar 2014 and first published on 19 Mar 2014


Article type: Paper
DOI: 10.1039/C4SM00410H
Citation: Soft Matter, 2014,10, 4268-4274
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    Local and transient permeation events are associated with local melting of giant liposomes

    T. Andersen, A. Kyrsting and P. M. Bendix, Soft Matter, 2014, 10, 4268
    DOI: 10.1039/C4SM00410H

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