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Issue 5, 2014
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Metal-catalyzed uncaging of DNA-binding agents in living cells

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Abstract

Attachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds.

Graphical abstract: Metal-catalyzed uncaging of DNA-binding agents in living cells

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The article was received on 03 Dec 2013, accepted on 30 Jan 2014 and first published on 31 Jan 2014


Article type: Edge Article
DOI: 10.1039/C3SC53317D
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Citation: Chem. Sci., 2014,5, 1901-1907
  • Open access: Creative Commons BY-NC license
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    Metal-catalyzed uncaging of DNA-binding agents in living cells

    M. I. Sánchez, C. Penas, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2014, 5, 1901
    DOI: 10.1039/C3SC53317D

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