Issue 8, 2015

A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications

Abstract

3-Epideacetycinobufagin (EDCB) was found to be a highly isoform-specific probe substrate for 3-glucuronidation mediated by UDP-glucuronosyltransferase 2B7 (UGT2B7). The probe reaction was well-characterized, and our results strongly suggest that EDCB can be used specially to measure the catalytic activity of UGT2B7 in biological samples.

Graphical abstract: A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications

Supplementary files

Article information

Article type
Communication
Submitted
05 Sep 2014
Accepted
26 Nov 2014
First published
27 Nov 2014

RSC Adv., 2015,5, 5924-5927

A highly selective probe for UDP-glucuronosyltransferase 2B7 (UGT2B7) in human microsomes: isoform specificity, kinetic characterization, and applications

X. Tian, C. Wang, G. Ge, J. Ning, C. Ai, J. Y. Hong, Y. Cai, X. Huo, J. Hou, K. Liu, H. Sun and X. Ma, RSC Adv., 2015, 5, 5924 DOI: 10.1039/C4RA09819F

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