DNA aptamer-based surface plasmon resonance sensing of human C-reactive protein

Abstract

Human C-reactive protein (CRP) is a powerful predictor and risk factor of inflammation and cardiovascular disease. We have obtained high-affinity DNA aptamers selected by an immobilization-free screening method, and the lowest K_d value of the aptamers is 3.9 nM. The affinity and specificity of the aptamers have been further investigated using aptamer-based SPR analysis, which will be of benefit for constructing DNA aptamer-based sensors for CRP detection.