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Issue 57, 2014
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Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

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Abstract

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.

Graphical abstract: Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

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Publication details

The article was received on 05 Feb 2014, accepted on 27 Jun 2014 and first published on 27 Jun 2014


Article type: Communication
DOI: 10.1039/C4RA01027B
Citation: RSC Adv., 2014,4, 30462-30466
  • Open access: Creative Commons BY license
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    Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

    A. Raulf, C. K. Spahn, P. J. M. Zessin, K. Finan, S. Bernhardt, A. Heckel and M. Heilemann, RSC Adv., 2014, 4, 30462
    DOI: 10.1039/C4RA01027B

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