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Issue 8, 2014
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Photophysics and ex vivo biodistribution of β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications

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Abstract

Low aqueous solubility of porphyrin-based photosensitizers hampers their clinical use in photodynamic therapy because of complex delivery. In this study, we explore meso-tetra(m-hydroxyphenyl)-21,23H-porphyrin (mTHPP), a potent photosensitizer, covalently attached to β-cyclodextrin (CD-mTHPP) with a focus on topical delivery and cellular uptake. The photophysical properties of CD-mTHPP were examined using steady-state fluorescence and lifetime measurements verifying increased aqueous solubility. Confocal and fluorescence lifetime imaging microscopy on human squamous carcinoma cells (A431) evidenced a cytoplasmic uptake of CD-mTHPP in predominantly monomeric form. CD-mTHPP was also delivered to human skin ex vivo and the skin penetration was assessed using two-photon fluorescence microscopy. The results indicated that CD-mTHPP exhibits improved skin distribution compared to mTHPP alone using aqueous vehicles. Thus the CD-mTHPP conjugate demonstrates improved biodistribution ex vivo compared to mTHPP and is a promising multimodal system for photodynamic therapy.

Graphical abstract: Photophysics and ex vivo biodistribution of β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications

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Publication details

The article was received on 11 Mar 2014, accepted on 09 Jun 2014 and first published on 10 Jun 2014


Article type: Paper
DOI: 10.1039/C4PP00088A
Author version available: Download Author version (PDF)
Citation: Photochem. Photobiol. Sci., 2014,13, 1185-1191
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    Photophysics and ex vivo biodistribution of β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications

    V. Kirejev, A. R. Gonçalves, C. Aggelidou, I. Manet, J. Mårtensson, K. Yannakopoulou and M. B. Ericson, Photochem. Photobiol. Sci., 2014, 13, 1185
    DOI: 10.1039/C4PP00088A

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