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Issue 22, 2014
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High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

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Abstract

High precision, high yield, and high density self-assembly of nanoparticles into arrays is essential for nanophotonics. Spatial deviations as small as a few nanometers can alter the properties of near-field coupled optical nanostructures. Several studies have reported assemblies of few nanoparticle structures with controlled spacing using DNA nanostructures with variable yield. Here, we report multi-tether design strategies and attachment yields for homo- and hetero-nanoparticle arrays templated by DNA origami nanotubes. Nanoparticle attachment yield via DNA hybridization is comparable with streptavidin-biotin binding. Independent of the number of binding sites, >97% site-occupation was achieved with four tethers and 99.2% site-occupation is theoretically possible with five tethers. The interparticle distance was within 2 nm of all design specifications and the nanoparticle spatial deviations decreased with interparticle spacing. Modified geometric, binomial, and trinomial distributions indicate that site-bridging, steric hindrance, and electrostatic repulsion were not dominant barriers to self-assembly and both tethers and binding sites were statistically independent at high particle densities.

Graphical abstract: High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

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Publication details

The article was received on 04 Jun 2014, accepted on 06 Oct 2014 and first published on 07 Oct 2014


Article type: Paper
DOI: 10.1039/C4NR03069A
Citation: Nanoscale, 2014,6, 13928-13938
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    High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

    S. Takabayashi, W. P. Klein, C. Onodera, B. Rapp, J. Flores-Estrada, E. Lindau, L. Snowball, J. T. Sam, J. E. Padilla, J. Lee, W. B. Knowlton, E. Graugnard, B. Yurke, W. Kuang and W. L. Hughes, Nanoscale, 2014, 6, 13928
    DOI: 10.1039/C4NR03069A

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