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Issue 21, 2014
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Effects of nanopillar array diameter and spacing on cancer cell capture and cell behaviors

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Abstract

While substrates with nanopillars (NPs) have emerged as promising platforms for isolation of circulating tumor cells (CTCs), the influence of diameter and spacing of NPs on CTC capture is still unclear. In this paper, CTC-capture yield and cell behaviors have been investigated by using antibody functionalized NPs of various diameters (120–1100 nm) and spacings (35–800 nm). The results show a linear relationship between the cell capture yield and effective contact area of NP substrates where a NP array of small diameter and reasonable spacing is preferred; however, spacing that is too small or too large adversely impairs the capture efficiency and specificity, respectively. In addition, the formation of pseudopodia between captured cells and the substrate is found to be dependent not only on cell adhesion status but also on elution strength and shear direction. These findings provide essential guidance in designing NP substrates for more efficient capture of CTCs and manipulation of cytomorphology in future.

Graphical abstract: Effects of nanopillar array diameter and spacing on cancer cell capture and cell behaviors

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Publication details

The article was received on 24 May 2014, accepted on 16 Jul 2014 and first published on 29 Jul 2014


Article type: Paper
DOI: 10.1039/C4NR02854F
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2014,6, 12482-12489
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    Effects of nanopillar array diameter and spacing on cancer cell capture and cell behaviors

    S. Wang, Y. Wan and Y. Liu, Nanoscale, 2014, 6, 12482
    DOI: 10.1039/C4NR02854F

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