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Issue 15, 2014
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Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

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Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport.

Graphical abstract: Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

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Publication details

The article was received on 11 Apr 2014, accepted on 04 Jun 2014 and first published on 06 Jun 2014


Article type: Communication
DOI: 10.1039/C4NR01951B
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2014,6, 8521-8526
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    Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

    J. Qi, J. Zeng, F. Zhao, S. H. Lin, B. Raja, U. Strych, R. C. Willson and W. Shih, Nanoscale, 2014, 6, 8521
    DOI: 10.1039/C4NR01951B

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