A mechanistic study on the α-N-acetylgalactosaminidase from E. meningosepticum: a family 109 glycoside hydrolase

Abstract

A recombinant glycoside hydrolase family 109 α-N-acetylgalactosaminidase from the pathogenic bacteria E. meningosepticum catalyses the hydrolysis of aryl 2-acetamido-2-deoxy-α-D-galactopyranosides. The sensitivities to leaving group abilities (β_lg values) on V and V/K are −0.08 ± 0.06 and −0.31 ± 0.12, respectively. These results are consistent with an E2 elimination following hydride transfer from C3.