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Issue 10, 2015
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Probing the epigenetic regulation of HIF-1α transcription in developing tissue

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Abstract

HIF-1 is the master regulator of cellular hypoxia response; the oxygen sensitive HIF-1α subunit transactivates its own expression in hypoxia via a hypoxia response element (HRE) in the promoter of the HIF-1α gene. This transactivation loop significantly contributes to the build up of HIF-1α at the onset of hypoxia, with the binding of HIF-1 to the HIF-1α promoter being dependent on the epigenetic status of a CpG dinucleotide in the upstream HRE. Given the central role played by HIF-1 in tissue development, we sought to probe the epigenetic status of the HIF-1α HRE and that of its downstream target EPO in embryonic tissue. Our data shows that the CpG dinucleotide in HIF-1α HRE is unmethylated in several embryonic tissue samples, suggesting that transactivation of HIF-1α plays a significant role in HIF-1 mediated hypoxia response during development.

Graphical abstract: Probing the epigenetic regulation of HIF-1α transcription in developing tissue

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Publication details

The article was received on 21 Apr 2015, accepted on 04 Jun 2015 and first published on 04 Jun 2015


Article type: Paper
DOI: 10.1039/C5MB00281H
Author version available: Download Author version (PDF)
Citation: Mol. BioSyst., 2015,11, 2780-2785
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    Probing the epigenetic regulation of HIF-1α transcription in developing tissue

    I. N. Mistry, P. J. S. Smith, D. I. Wilson and A. Tavassoli, Mol. BioSyst., 2015, 11, 2780
    DOI: 10.1039/C5MB00281H

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