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Issue 19, 2014
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Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

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Abstract

We describe a versatile microfluidic fluorescence-activated cell sorter that uses acoustic actuation to sort cells or drops at ultra-high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell (FACS) and droplet sorting (FADS) and is applicable for a multitude of objects. We sort aqueous droplets, at rates as high as several kHz, into two or even more outlet channels. We can also sort cells directly from the medium without prior encapsulation into drops; we demonstrate this by sorting fluorescently labeled mouse melanoma cells in a single phase fluid. Our acoustic microfluidic FACS is compatible with standard cell sorting cytometers, yet, at the same time, enables a rich variety of more sophisticated applications.

Graphical abstract: Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

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Publication details

The article was received on 20 May 2014, accepted on 30 Jun 2014 and first published on 30 Jun 2014


Article type: Paper
DOI: 10.1039/C4LC00588K
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 3710-3718
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    Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

    L. Schmid, D. A. Weitz and T. Franke, Lab Chip, 2014, 14, 3710
    DOI: 10.1039/C4LC00588K

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