Jump to main content
Jump to site search

Issue 16, 2014
Previous Article Next Article

A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood

Author affiliations

Abstract

We present a new concept for rapid and fully portable prostate specific antigen (PSA) measurements, termed “lab-in-a-briefcase”, which integrates an affordable microfluidic ELISA platform utilising a melt-extruded fluoropolymer microcapillary film (MCF) containing an array of 10 200 μm internal diameter capillaries, a disposable multi-syringe aspirator (MSA), a sample tray pre-loaded with all of the required immunoassay reagents, and a portable film scanner for colorimetric signal digital quantification. Each MSA can perform 10 replicate microfluidic immunoassays on 8 samples, allowing 80 measurements to be made in less than 15 minutes based on semi-automated operation, without the need of additional fluid handling equipment. The assay was optimised for the measurement of a clinically relevant range of PSA of 0.9 to 60.0 ng ml−1 in 15 minutes with CVs on the order of 5% based on intra-assay variability when read using a consumer flatbed film scanner. The PSA assay performance in the MSA remained robust in undiluted or 1 : 2 diluted human serum or whole blood, and the matrix effect could simply be overcome by extending sample incubation times. The PSA “lab-in-a-briefcase” is particularly suited to a low-resource health setting, where diagnostic labs and automated immunoassay systems are not accessible, by allowing PSA measurement outside the laboratory using affordable equipment.

Graphical abstract: A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Apr 2014, accepted on 09 Jun 2014 and first published on 10 Jun 2014


Article type: Paper
DOI: 10.1039/C4LC00464G
Citation: Lab Chip, 2014,14, 2918-2928
  • Open access: Creative Commons BY license
  •   Request permissions

    A lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood

    A. I. Barbosa, A. P. Castanheira, A. D. Edwards and N. M. Reis, Lab Chip, 2014, 14, 2918
    DOI: 10.1039/C4LC00464G

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements