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Issue 17, 2014
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Microfluidic cryofixation for correlative microscopy

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Abstract

Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.

Graphical abstract: Microfluidic cryofixation for correlative microscopy

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Publication details

The article was received on 17 Mar 2014, accepted on 30 Jun 2014 and first published on 30 Jun 2014


Article type: Communication
DOI: 10.1039/C4LC00333K
Citation: Lab Chip, 2014,14, 3281-3284
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    Microfluidic cryofixation for correlative microscopy

    Y. X. Mejia, H. Feindt, D. Zhang, S. Steltenkamp and T. P. Burg, Lab Chip, 2014, 14, 3281
    DOI: 10.1039/C4LC00333K

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