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Issue 11, 2014
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Micropillar arrays enabling single microbial cell encapsulation in hydrogels

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Abstract

Single microbial cell encapsulation in hydrogels is an important task to find valuable biological resources for human welfare. The conventional microfluidic designs are mainly targeted only for highly dispersed spherical bioparticles. Advanced structures should be taken into consideration for handling such aggregated and non-spherical microorganisms. Here, to address the challenge, we propose a new type of cylindrical-shaped micropillar array in a microfluidic device for enhancing the dispersion of cell clusters and the isolation of individual cells into individual micro-hydrogels for potential practical applications. The incorporated micropillars act as a sieve for the breaking of Escherichia coli (E. coli) clusters into single cells in a polymer mixture. Furthermore, the combination of hydrodynamic forces and a flow-focusing technique will improve the probability of encapsulation of a single cell into each hydrogel with a broad range of cell concentrations. This proposed strategy and device would be a useful platform for genetically modified microorganisms for practical applications.

Graphical abstract: Micropillar arrays enabling single microbial cell encapsulation in hydrogels

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Publication details

The article was received on 16 Jan 2014, accepted on 18 Feb 2014 and first published on 18 Feb 2014


Article type: Paper
DOI: 10.1039/C4LC00070F
Citation: Lab Chip, 2014,14, 1873-1879
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    Micropillar arrays enabling single microbial cell encapsulation in hydrogels

    K. J. Park, K. G. Lee, S. Seok, B. G. Choi, M. Lee, T. J. Park, J. Y. Park, D. H. Kim and S. J. Lee, Lab Chip, 2014, 14, 1873
    DOI: 10.1039/C4LC00070F

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