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Issue 8, 2014
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Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

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Abstract

Continuous flow particulate-based microfluidic processors are in critical demand for emerging applications in chemistry and biology, such as point-of-care molecular diagnostics. Challenges remain, however, for accomplishing biochemical assays in which microparticle immobilization is desired or required during intermediate stages of fluidic reaction processes. Here we present a dual-mode microfluidic reactor that functions autonomously under continuous flow conditions to: (i) execute multi-stage particulate-based fluidic mixing routines, and (ii) array select numbers of microparticles during each reaction stage (e.g., for optical detection). We employ this methodology to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-stage aptamer-based sandwich assay.

Graphical abstract: Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

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Publication details

The article was received on 05 Jan 2014, accepted on 12 Feb 2014 and first published on 12 Feb 2014


Article type: Technical Innovation
DOI: 10.1039/C4LC00012A
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 1405-1409
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    Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

    R. D. Sochol, D. Corbett, S. Hesse, W. E. R. Krieger, K. T. Wolf, M. Kim, K. Iwai, S. Li, L. P. Lee and L. Lin, Lab Chip, 2014, 14, 1405
    DOI: 10.1039/C4LC00012A

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