Issue 9, 2014

Laser-induced microbubble poration of localized single cells

Abstract

Laser-induced microbubbles were used to porate the cell membranes of localized single NIH/3T3 fibroblasts. Microsecond laser pulses were focused on an optically absorbent substrate, creating a vapour microbubble that oscillated in size at the laser focal point in a fluidic chamber. The shear stress accompanying the bubble size oscillation was able to porate nearby cells. Cell poration was demonstrated with the delivery of FITC–dextran dye with various molecular weights. Under optimal poration conditions, the cell poration efficiency was up to 95.2 ± 4.8%, while maintaining 97.6 ± 2.4% cell viability. The poration system is able to target a single cell without disturbing surrounding cells.

Graphical abstract: Laser-induced microbubble poration of localized single cells

Supplementary files

Article information

Article type
Paper
Submitted
17 Dec 2013
Accepted
06 Feb 2014
First published
06 Feb 2014

Lab Chip, 2014,14, 1572-1578

Author version available

Laser-induced microbubble poration of localized single cells

Q. Fan, W. Hu and A. T. Ohta, Lab Chip, 2014, 14, 1572 DOI: 10.1039/C3LC51394G

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