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Issue 8, 2014
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Micro-patterning of mammalian cells on suspended MEMS resonant sensors for long-term growth measurements

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Abstract

MEMS resonant mass sensors can measure the mass of individual cells, though long-term growth measurements are limited by the movement of cells off the sensor area. Micro-patterning techniques are a powerful approach to control the placement of individual cells in an arrayed format. In this work we present a method for micro-patterning cells on fully suspended resonant sensors through select functionalization and passivation of the chip surface. This method combines high-resolution photolithography with a blanket transfer technique for applying photoresist to avoid damaging the sensors. Cells are constrained to the patterned collagen area on the sensor by pluronic acting as a cell adhesion blocker. This micro-patterning method enables long-term growth measurements, which is demonstrated by a measurement of the change in mass of a human breast cancer cell over 18 h.

Graphical abstract: Micro-patterning of mammalian cells on suspended MEMS resonant sensors for long-term growth measurements

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Publication details

The article was received on 28 Oct 2013, accepted on 04 Feb 2014 and first published on 04 Feb 2014


Article type: Technical Innovation
DOI: 10.1039/C3LC51217G
Citation: Lab Chip, 2014,14, 1401-1404
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    Micro-patterning of mammalian cells on suspended MEMS resonant sensors for long-term growth measurements

    E. A. Corbin, B. R. Dorvel, L. J. Millet, W. P. King and R. Bashir, Lab Chip, 2014, 14, 1401
    DOI: 10.1039/C3LC51217G

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