Issue 22, 2014

Development of a functional ruthenium(ii) complex for probing hypochlorous acid in living cells

Abstract

A functional ruthenium(II) complex, [Ru(bpy)2(AN-bpy)](PF6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(II) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy)2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl-4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(II) complex probe, [Ru(bpy)2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.

Graphical abstract: Development of a functional ruthenium(ii) complex for probing hypochlorous acid in living cells

Supplementary files

Article information

Article type
Paper
Submitted
18 Jan 2014
Accepted
08 Mar 2014
First published
10 Mar 2014

Dalton Trans., 2014,43, 8414-8420

Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

Z. Ye, R. Zhang, B. Song, Z. Dai, D. Jin, E. M. Goldys and J. Yuan, Dalton Trans., 2014, 43, 8414 DOI: 10.1039/C4DT00179F

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