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Issue 25, 2015
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A mild TCEP-based para-azidobenzyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates

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Abstract

It has recently emerged that the succinimide linkage of a maleimide thiol addition product is fragile, which is a major issue in fields where thiol functionalisation needs to be robust. Herein we deliver a strategy that generates selective cysteine thiol labelling reagents, which are stable to hydrolysis and thiol exchange.

Graphical abstract: A mild TCEP-based para-azidobenzyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates

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Publication details

The article was received on 28 Oct 2014, accepted on 12 Nov 2014 and first published on 12 Nov 2014


Article type: Communication
DOI: 10.1039/C4CC08515A
Citation: Chem. Commun., 2015,51, 5279-5282
  • Open access: Creative Commons BY license
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    A mild TCEP-based para-azidobenzyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates

    A. Maruani, S. Alom, P. Canavelli, M. T. W. Lee, R. E. Morgan, V. Chudasama and S. Caddick, Chem. Commun., 2015, 51, 5279
    DOI: 10.1039/C4CC08515A

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