Issue 100, 2014
From the journal:

Chemical Communications

Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1†

Shunbi Xie,a   Yali Yuan,a   Yue Song,a   Ying Zhuo,a   Tian Li,a   Yaqin Chai*a  and  Ruo Yuan*a  

* Corresponding authors

a State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China
E-mail: yuanruo@swu.edu.cn, yqchai@swu.edu.cn
Fax: +86-23-68253172
Tel: +86-23-68252277

Abstract

Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during the loop mediated isothermal amplification (LAMP) procedure by using a pH meter. The genomic DNA of Nosema bombycis (N. bombycis) was amplified and detected by employing this LAMP-pH meter platform for the first time.

Graphical abstract: Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1

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Article information

DOI
https://doi.org/10.1039/C4CC06449F
Article type
Communication
Submitted
16 Aug 2014
Accepted
24 Oct 2014
First published
24 Oct 2014

Chem. Commun., 2014,50, 15932-15935

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Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1

S. Xie, Y. Yuan, Y. Song, Y. Zhuo, T. Li, Y. Chai and R. Yuan, Chem. Commun., 2014, 50, 15932 DOI: 10.1039/C4CC06449F

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