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Issue 38, 2014
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A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

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Abstract

A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible site-specific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.

Graphical abstract: A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

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Publication details

The article was received on 28 Feb 2014, accepted on 21 Mar 2014 and first published on 21 Mar 2014


Article type: Communication
DOI: 10.1039/C4CC01528B
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Citation: Chem. Commun., 2014,50, 4891-4894
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    A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

    H. Zhang, Y. Song, Y. Zou, Y. Ge, Y. An, Y. Ma, Z. Zhu and C. J. Yang, Chem. Commun., 2014, 50, 4891
    DOI: 10.1039/C4CC01528B

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