Issue 11, 2014
From the journal:

Chemical Communications

An intracellular buildup reaction of active siRNA species from short RNA fragments†

Hideto Maruyama,ab   Yuko Nakashima,a   Satoshi Shuto,b   Akira Matsuda,b   Yoshihiro Ito*ac  and  Hiroshi Abe*abcd  

* Corresponding authors

a Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan
E-mail: h-abe@pharm.hokuadai.ac.jp, y-ito@riken.jp

b Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan

c Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

d PRESTO, Japan Science and Technology Agency, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

Here we report a new strategy for the buildup reaction of active siRNA species from short RNA fragments in living cells using a chemical ligation reaction. This strategy could decrease undesired immune responses and provide more latitude for RNAi technology in the design and concentration of introduced RNA compared to traditional siRNA methods.

Graphical abstract: An intracellular buildup reaction of active siRNA species from short RNA fragments

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Article information

DOI
https://doi.org/10.1039/C3CC47529H
Article type
Communication
Submitted
01 Oct 2013
Accepted
28 Nov 2013
First published
29 Nov 2013

Chem. Commun., 2014,50, 1284-1287

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An intracellular buildup reaction of active siRNA species from short RNA fragments

H. Maruyama, Y. Nakashima, S. Shuto, A. Matsuda, Y. Ito and H. Abe, Chem. Commun., 2014, 50, 1284 DOI: 10.1039/C3CC47529H

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