Issue 11, 2014

Stem cell culture using cell-derived substrates

Abstract

There have been great efforts to develop cell culture systems to regulate stem cell functions. Development of cell culture substrates is one of the important approaches for stem cell culture because substrates influence stem cell functions such as attachment, proliferation, self-renewal, and induction of differentiation. Stem cells are surrounded by their specific microenvironments in vivo, composed of cells, cytokines, and an extracellular matrix (ECM), which may dynamically change and affect cellular activities accordingly. To mimic such microenvironments, cell culture substrates can be prepared by coating bioactive proteins such as ECM proteins and signaling molecules as ligands for cell surface receptors. Compared with protein-coated substrates, cell- and cell-formed ECM-derived substrates have shown great progress and attracted significant attention as functional and prospective biomaterials for stem cell culture and regenerative medicine. In this review, we summarize the latest progress of these new substrates derived from cells and cell-formed ECMs.

Graphical abstract: Stem cell culture using cell-derived substrates

Article information

Article type
Minireview
Submitted
15 Apr 2014
Accepted
24 Jul 2014
First published
08 Aug 2014

Biomater. Sci., 2014,2, 1595-1603

Author version available

Stem cell culture using cell-derived substrates

B. Joddar, T. Hoshiba, G. Chen and Y. Ito, Biomater. Sci., 2014, 2, 1595 DOI: 10.1039/C4BM00126E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements