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Issue 16, 2014
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Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

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Abstract

In the present study, gene analysis using siRNAs for cell differentiation of embryonic stem (ES) cells was performed using an LSI-based amperometric sensor array (Bio-LSI). CITED2 and WNT11 were silenced using siRNA, and then the effect of these genes on the differentiation of ES cells into cardiomyocytes was evaluated. For the evaluation, endogenous ALP activity and sarcomeric α-actinin were electrochemically detected using the Bio-LSI system. These results show the crucial role of these genes in determining the fate of differentiation of ES cells. These results also show that the Bio-LSI is a great tool for cell analysis.

Graphical abstract: Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

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Publication details

The article was received on 01 Apr 2014, accepted on 10 Apr 2014 and first published on 11 Apr 2014


Article type: Paper
DOI: 10.1039/C4AY00791C
Citation: Anal. Methods, 2014,6, 6337-6342
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    Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

    M. Şen, K. Ino, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, H. Shiku and T. Matsue, Anal. Methods, 2014, 6, 6337
    DOI: 10.1039/C4AY00791C

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