Molecular modelling and synthesis of a polymer for the extraction of amiloride and triamterene from human urine

Abstract

A new solid-phase extraction resin with high affinity towards amiloride and triamterene was developed with the assistance of computational modelling. Itaconic acid was selected as the best functional monomer capable of forming strong complexes with amiloride and triamterene. Conditions for selective solid-phase extraction of the diuretics from human urine and for their separation using a custom-designed adsorbent were optimised. It was shown that this adsorbent provided a high level of purification of the diuretics from human urine, which made their quantification using spectrophotometry possible.