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Issue 2, 2014
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PCR – the polymerase chain reaction


DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied. Mullis's invention allowed researchers to make millions of copies of a selected DNA region within hours. Today, PCR can take minutes. PCR has widespread analytical applications in the food, environmental, medical and forensic fields. This Technical Brief covers the basics of PCR and some of its variations.

Graphical abstract: PCR – the polymerase chain reaction

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Publication details

The article was received on 19 Nov 2013, accepted on 19 Nov 2013 and first published on 10 Dec 2013

Article type: AMC Technical Brief
DOI: 10.1039/C3AY90101G
Citation: Anal. Methods, 2014,6, 333-336
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    PCR – the polymerase chain reaction

    Analytical Methods Committee, AMCTB No 59, Anal. Methods, 2014, 6, 333
    DOI: 10.1039/C3AY90101G

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