Issue 1, 2015

Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells

Abstract

A new type of resorufin-based dual-functional fluorescent probe whose fluorescence emission features are sensitive to thiol compounds and redox homeostasis was developed. Thiols-triggered nucleophilic substitution of the probes converts the nonfluorescent probe to the highly fluorescent resorufin moiety; the released resorufin not only enables fluorescence signaling specific for thiol compounds but functions as a redox indicator with sensitive colorimetric and fluorescence emission change upon redox variation. Preliminary fluorescence imaging experiments have revealed the biocompatibility of the as-prepared probes and validated their practicability for thiol sensing and redox homeostasis mapping in living cells.

Graphical abstract: Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells

Article information

Article type
Paper
Submitted
06 Aug 2014
Accepted
22 Oct 2014
First published
23 Oct 2014

Analyst, 2015,140, 322-329

Author version available

Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells

T. Ma, H. Ding, H. Xu, Y. Lv, H. Liu, H. Wang and Z. Tian, Analyst, 2015, 140, 322 DOI: 10.1039/C4AN01441C

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