Jump to main content
Jump to site search

Issue 21, 2014
Previous Article Next Article

Switching specific biomolecular interactions on surfaces under complex biological conditions

Author affiliations

Abstract

Herein, electrically switchable mixed self-assembled monolayers based on oligopeptides have been developed and investigated for their suitability in achieving control over biomolecular interactions in the presence of complex biological conditions. Our model system, a biotinylated oligopeptide tethered to gold within a background of tri(ethylene glycol) undecanethiol, is ubiquitous in both switching specific protein interactions in highly fouling media while still offering the non-specific protein-resistance to the surface. Furthermore, the work demonstrated that the performance of the switching on the electro-switchable oligopeptide is sensitive to the characteristics of the media, and in particular, its protein concentration and buffer composition, and thus such aspects should be considered and addressed to assure maximum switching performance. This study lays the foundation for developing more realistic dynamic extracellular matrix models and is certainly applicable in a wide variety of biological and medical applications.

Graphical abstract: Switching specific biomolecular interactions on surfaces under complex biological conditions

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Jul 2014, accepted on 21 Aug 2014 and first published on 21 Aug 2014


Article type: Paper
DOI: 10.1039/C4AN01225A
Citation: Analyst, 2014,139, 5400-5408
  • Open access: Creative Commons BY license
  •   Request permissions

    Switching specific biomolecular interactions on surfaces under complex biological conditions

    M. Lashkor, F. J. Rawson, J. A. Preece and P. M. Mendes, Analyst, 2014, 139, 5400
    DOI: 10.1039/C4AN01225A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements