Issue 17, 2014

Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

Abstract

An aptamer-based competitive binding assay for one-step (i.e. no requirement of pre-treatment) quantitation of target molecules of interest has been developed. This method has been successfully employed for the fast and sensitive detection of the surface antigen of the hepatitis B virus (HBsAg). The key features of our method include its low intrinsic background noise, low costs, high resolution, and high sensitivity, enabling the detection of as low as 1.25 mIU mL−1, approximately 40-fold better than that of the most widely used Abbott Architect assay for HBsAg detection, without the tedious extraction and/or washing procedures. Moreover, this assay has better recovery and accuracy than that of conventional competitive binding assay or others for HBsAg quantitation.

Graphical abstract: Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

Supplementary files

Article information

Article type
Paper
Submitted
06 Apr 2014
Accepted
11 Jun 2014
First published
11 Jun 2014

Analyst, 2014,139, 4310-4314

Author version available

Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

S. Suh, S. Song, H. Oh, S. Hwang and S. S. Hah, Analyst, 2014, 139, 4310 DOI: 10.1039/C4AN00619D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements