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Issue 40, 2013
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Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface

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Abstract

Recently, the transfer method has been shown to be useful for preparing cell-sized phospholipid bilayer vesicles, within which desired substances at desired concentrations can be encapsulated, with a desired asymmetric lipid composition. Here, we investigated the transfer process of water-in-oil (W/O) droplets coated by phospholipid monolayers across an oil/water interface by both experimental observation and theoretical modeling. Real-time experimental observation of the transfer revealed that the transfer process is characterized by three kinetic regimes: a precontact process (approaching regime), an early fast process (entering regime), and a late slow process (relaxation regime). In addition, bigger droplets require much more time to transfer than smaller droplets. We propose a theoretical model to interpret this kinetic process. Our theoretical model reproduces the essential aspects of the transfer kinetics, including its size-dependence.

Graphical abstract: Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface

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Publication details

The article was received on 27 Jun 2013, accepted on 02 Aug 2013 and first published on 07 Aug 2013


Article type: Paper
DOI: 10.1039/C3SM51766G
Citation: Soft Matter, 2013,9, 9539-9547
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    Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface

    H. Ito, T. Yamanaka, S. Kato, T. Hamada, M. Takagi, M. Ichikawa and K. Yoshikawa, Soft Matter, 2013, 9, 9539
    DOI: 10.1039/C3SM51766G

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