Issue 20, 2013

Deformation and poration of lipid bilayer membranes by cationic nanoparticles

Abstract

Synthetic lipid bilayers in a giant unilamellar vesicle (GUV) format were used to study potentially harmful interactions between nanoparticles and biomembranes. Confocal fluorescence microscopy shows that 20 nm polystyrene nanoparticles with cationic surfaces adhere strongly to these lipid bilayer membranes. This adhesion is sensitive to the ionic strength of the surrounding medium and is independent of the lipid composition of the membrane, indicating that it is driven by nonspecific electrostatic interactions. Nanoparticles bound to the GUV membrane form extended protrusions that incorporate the membrane lipids. As the membrane material is pulled into these protrusions, the diameter of the GUV shrinks. This process is accompanied by the formation of transient pores in the membrane, as indicated by the leakage of high-molecular weight polymers from the GUV interior. There is an upper limit to the molecular weight of polymer that will leak through these pores; based on this size limit, the pore diameter can be estimated as 18–27 nm. These results suggest that nanoparticle adhesion imposes surface tension on biomembranes via a steric crowding mechanism, leading to poration. The phenomenon is potentially a physiologically relevant mode of interaction between nanoparticles and biomembranes, and may help explain observed plasma membrane permeabilization in the presence of broad classes of nanoparticles.

Graphical abstract: Deformation and poration of lipid bilayer membranes by cationic nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
08 Nov 2012
Accepted
13 Mar 2013
First published
27 Mar 2013

Soft Matter, 2013,9, 4969-4976

Deformation and poration of lipid bilayer membranes by cationic nanoparticles

S. Li and N. Malmstadt, Soft Matter, 2013, 9, 4969 DOI: 10.1039/C3SM27578G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements