Issue 7, 2013

Dynamic and bio-orthogonal proteinassembly along a supramolecular polymer

Abstract

Dynamic protein assembly along supramolecular columnar polymers has been achieved through the site-specific covalent attachment of different SNAP-tag fusion proteins to self-assembled benzylguanine-decorated discotics. The self-assembly of monovalent discotics into supramolecular polymers creates a multivalent, bio-orthogonal and self-regulating framework for protein assembly. The intrinsic reversibility of supramolecular interactions results in reorganization and exchange of building blocks allowing for dynamic intermixing of protein-functionalized discotics between different self-assembled polymers, leading to self-optimization of protein arrangement and distance as evidenced by efficient energy transfer between fluorescent proteins.

Graphical abstract: Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

Supplementary files

Article information

Article type
Edge Article
Submitted
03 Apr 2013
Accepted
26 Apr 2013
First published
26 Apr 2013
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2013,4, 2886-2891

Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

K. Petkau-Milroy, D. A. Uhlenheuer, A. J. H. Spiering, J. A. J. M. Vekemans and L. Brunsveld, Chem. Sci., 2013, 4, 2886 DOI: 10.1039/C3SC50891A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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