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Issue 3, 2013
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A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells

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Abstract

Although labile iron plays critical roles in diverse biological processes in living cells, the physiological and pathophysiological functions of iron have not been sufficiently explored, partially due to a lack of methods for visualizing intracellular labile iron. In this edge article, we present a novel turn-on fluorescent probe (RhoNox-1) for the selective detection of Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations and electrochemical studies revealed that fluorescence quenching of RhoNox-1 occurred in physiological conditions, which was attributed to non-radiative deactivation of the excited state of tertiary amine N-oxide substituted xanthene involving a twisted internal charge transfer (TICT) process and partially due to photo-induced electron transfer (PET) from the N-oxide group. RhoNox-1 showed significant enhancement of the fluorescence signal in Fe2+-loaded cells via selective Fe2+-mediated deoxygenation of the N-oxide group and also successfully detected basal and endogenous labile Fe2+ in living cells.

Graphical abstract: A highly selective turn-on fluorescent probe for iron(ii) to visualize labile iron in living cells

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Publication details

The article was received on 03 Oct 2012, accepted on 10 Dec 2012, published on 20 Dec 2012 and first published online on 20 Dec 2012


Article type: Edge Article
DOI: 10.1039/C2SC21649C
Citation: Chem. Sci., 2013,4, 1250-1256
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    A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells

    T. Hirayama, K. Okuda and H. Nagasawa, Chem. Sci., 2013, 4, 1250
    DOI: 10.1039/C2SC21649C

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