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Issue 31, 2013
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Stimuli-responsive HA-PEI nanoparticles encapsulating endostatin plasmid for stem cell gene therapy

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Abstract

In this study, an environmentally-sensitive hyaluronic acid (HA)-polyethylenimine (PEI) copolymer with disulfide linkage is synthesized, characterized and examined as a potential non-viral gene vector. From the results, a redox- and pH-sensitive gene delivery nanocarrier has been successfully synthesized via the analysis of 1H NMR and FT-IR. The positive HA-ss-PEI conjugate complexed with negatively charged plasmid DNA can be achieved via electrostatic attraction to form a stable spherical nanoparticle of 100 to 200 nm in diameter. N/P = 2 possesses the optimal condition for releasing encapsulated plasmid when triggered by stimuli such as redox reaction and pH change. The HA-ss-PEI/pEndo nanocarrier can transfect human mesenchymal stem cells to produce endostatin protein and these hMSCs are capable of differentiating into functional chondrocytes after 7 days chondrogenic induction. The nano-encapsulation of genetic anti-angiogenesis factor combined with the chondrogenic induction of hMSCs may offer an alternative choice and potentially effective administration strategy for arthritis.

Graphical abstract: Stimuli-responsive HA-PEI nanoparticles encapsulating endostatin plasmid for stem cell gene therapy

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Publication details

The article was received on 20 Feb 2013, accepted on 24 Apr 2013 and first published on 01 May 2013


Article type: Paper
DOI: 10.1039/C3RA40880A
Citation: RSC Adv., 2013,3, 12922-12932
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    Stimuli-responsive HA-PEI nanoparticles encapsulating endostatin plasmid for stem cell gene therapy

    P. Yeh, J. Sun, H. Wu, L. Hwang and T. Wang, RSC Adv., 2013, 3, 12922
    DOI: 10.1039/C3RA40880A

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