Issue 43, 2013

Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

Abstract

A novel method for the parallel synthesis of peptide-biocargo conjugates was developed that utilizes affinity purification for fast isolation of the conjugates in order to avoid time consuming HPLC purification. The methodology was applied to create two libraries of cell-penetrating peptide (CPP)–PNA705 conjugates from parallel-synthesized peptide libraries. The conjugates were tested for their ability to induce splicing redirection in HeLa pLuc705 cells. The results demonstrate how the novel methodology can be applied for screening purposes in order to find suitable CPP-biocargo combinations and further optimization of CPPs.

Graphical abstract: Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

Supplementary files

Article information

Article type
Paper
Submitted
14 Aug 2013
Accepted
01 Oct 2013
First published
01 Oct 2013
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2013,11, 7621-7630

Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

P. J. Deuss, A. A. Arzumanov, D. L. Williams and M. J. Gait, Org. Biomol. Chem., 2013, 11, 7621 DOI: 10.1039/C3OB41659C

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