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Issue 44, 2013
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No washing, less waiting: engineering biomolecular reporters for single-step antibody detection in solution

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Abstract

Detection of antibodies is essential for the diagnosis of many disease states, including infectious diseases, autoimmune diseases and allergies. Most current antibody detection assays involve multistep detection schemes in which molecular recognition and signal generation are separate processes. A well-known example is the enzyme-linked immunosorbent assay (ELISA), which combines high sensitivity and specificity with strong signal amplification. However, ELISA and other heterogeneous methods require multiple, time-consuming washing and incubation steps, which limits their applicability in point-of-care diagnostics and high-throughput applications. In recent years, several new antibody detection strategies have been developed in which antibody binding and signal generation are integrated within a single biomolecular reporter. These strategies aim to rival ELISA in terms of sensitivity and specificity, while decreasing the time and effort required to perform an assay. Here, we review recent developments in this field according to their mechanism of action and discuss their advantages and limitations.

Graphical abstract: No washing, less waiting: engineering biomolecular reporters for single-step antibody detection in solution

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Publication details

The article was received on 26 Jun 2013, accepted on 10 Sep 2013 and first published on 04 Oct 2013


Article type: Perspective
DOI: 10.1039/C3OB41315B
Citation: Org. Biomol. Chem., 2013,11, 7642-7649
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    No washing, less waiting: engineering biomolecular reporters for single-step antibody detection in solution

    S. Banala, R. Arts, S. J. A. Aper and M. Merkx, Org. Biomol. Chem., 2013, 11, 7642
    DOI: 10.1039/C3OB41315B

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