Issue 31, 2013

Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex

Abstract

3-Cyanovinylcarbazole nucleoside (CNVK), which can quickly photocrosslink to the pyrimidine base in the DNA double strand, was incorporated into a triplex forming oligonucleotide (TFO). We successfully demonstrated that CNVK in TFO can photocrosslink to the thymine base in Watson–Crick double-stranded DNA via anti-Hoogsteen base pairing with a few seconds of photoirradiation.

Graphical abstract: Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex

Supplementary files

Article information

Article type
Communication
Submitted
02 May 2013
Accepted
24 May 2013
First published
24 May 2013

Org. Biomol. Chem., 2013,11, 5065-5068

Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex

K. Fujimoto, H. Yoshinaga, Y. Yoshio and T. Sakamoto, Org. Biomol. Chem., 2013, 11, 5065 DOI: 10.1039/C3OB40915E

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