Jump to main content
Jump to site search

Issue 21, 2013
Previous Article Next Article

Tracking the intracellular drug release from graphene oxide using surface-enhanced Raman spectroscopy

Author affiliations

Abstract

We have developed a graphene oxide (GO)-based nanoplatform simultaneously loaded with a chemical drug and Ag nanoparticles (NPs), and employed it to study the drug release from GO in living cells by surface-enhanced Raman spectroscopy (SERS). In our strategy, doxorubicin (DOX), a typical model anticancer drug, was loaded onto chemically prepared GO by means of π–π stacking, while the Ag NPs were covalently modified onto GO. After incubation of the DOX- and Ag NPs-loaded GO with Ca Ski cells for several hours, DOX will detach from the GO in an acidic environment due to the pH-dependent π–π interaction between DOX and GO. Real-time measurement of SERS signals of DOX using the GO loaded with Ag NPs as a SERS-active substrate allows us to monitor the process of the drug release inside the living cell. The SERS results reveal that DOX is initially released from the GO surface inside the lysosomes, then escapes into the cytoplasm, and finally enters the nucleus, while GO, the nanocarrier, remains within the cytoplasm, without entering the nucleus.

Graphical abstract: Tracking the intracellular drug release from graphene oxide using surface-enhanced Raman spectroscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Jun 2013, accepted on 28 Aug 2013 and first published on 29 Aug 2013


Article type: Paper
DOI: 10.1039/C3NR03264G
Citation: Nanoscale, 2013,5, 10591-10598
  •   Request permissions

    Tracking the intracellular drug release from graphene oxide using surface-enhanced Raman spectroscopy

    J. Huang, C. Zong, H. Shen, Y. Cao, B. Ren and Z. Zhang, Nanoscale, 2013, 5, 10591
    DOI: 10.1039/C3NR03264G

Search articles by author

Spotlight

Advertisements