Jump to main content
Jump to site search

Issue 11, 2013
Previous Article Next Article

The role of transcription in heterologous expression of polyketides in bacterial hosts

Author affiliations

Abstract

Heterologous expression of biosynthetic pathways is an indispensable tool in the discovery, production, engineering, and characterization of bacterial polyketides and the complex enzymology involved in their biosynthesis. Ensuring transcription of polyketide biosynthetic gene clusters in heterologous hosts is a pressing problem. This review evaluates the two strategies used to ensure transcription. The first is a promoter replacement approach where promoters known to function in the heterologous host are inserted into the biosynthetic gene cluster. The second is an approach that relies on the heterologous host recognizing and utilizing promoters native to the gene cluster. Both have been successful methodologies and have different strengths and weaknesses, which are highlighted and discussed.

Graphical abstract: The role of transcription in heterologous expression of polyketides in bacterial hosts

Back to tab navigation

Publication details

The article was received on 29 Jun 2013 and first published on 24 Sep 2013


Article type: Review Article
DOI: 10.1039/C3NP70060G
Citation: Nat. Prod. Rep., 2013,30, 1391-1411
  •   Request permissions

    The role of transcription in heterologous expression of polyketides in bacterial hosts

    D. C. Stevens, T. P. A. Hari and C. N. Boddy, Nat. Prod. Rep., 2013, 30, 1391
    DOI: 10.1039/C3NP70060G

Search articles by author

Spotlight

Advertisements