Jump to main content
Jump to site search

Issue 5, 2013
Previous Article Next Article

Preparation of non-aggregated fluorescent nanodiamonds (FNDs) by non-covalent coating with a block copolymer and proteins for enhancement of intracellular uptake

Author affiliations

Abstract

Fluorescent nanodiamonds (FNDs) are very promising fluorophores for use in biosystems due to their high biocompatibility and photostability. To overcome their tendency to aggregate in physiological solutions, which severely limits the biological applications of FNDs, we developed a new non-covalent coating method using a block copolymer, PEG-b-P(DMAEMA-co-BMA), or proteins such as BSA and HSA. By simple mixing of the block copolymer with FNDs, the cationic DMAEMA and hydrophobic BMA moieties can strongly interact with the anionic and hydrophobic moieties on the FND surface, while the PEG block can form a shell to prevent the direct contact between FNDs. The polymer-coated FNDs, along with BSA- and HSA-coated FNDs, showed non-aggregation characteristics and maintained their size at the physiological salt concentration. The well-dispersed, polymer- or protein-coated FNDs in physiological solutions showed enhanced intracellular uptake, which was confirmed by CLSM. In addition, the biocompatibility of the coated FNDs was expressly supported by a cytotoxicity assay. Our simple non-covalent coating with the block copolymer, which can be easily modified by various chemical methods, projects a very promising outlook for future biomedical applications, especially in comparison with covalent coating or protein-based coating.

Graphical abstract: Preparation of non-aggregated fluorescent nanodiamonds (FNDs) by non-covalent coating with a block copolymer and proteins for enhancement of intracellular uptake

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Oct 2012, accepted on 04 Jan 2013 and first published on 09 Jan 2013


Article type: Paper
DOI: 10.1039/C2MB25431J
Citation: Mol. BioSyst., 2013,9, 1004-1011
  •   Request permissions

    Preparation of non-aggregated fluorescent nanodiamonds (FNDs) by non-covalent coating with a block copolymer and proteins for enhancement of intracellular uptake

    J. W. Lee, S. Lee, S. Jang, K. Y. Han, Y. Kim, J. Hyun, S. K. Kim and Y. Lee, Mol. BioSyst., 2013, 9, 1004
    DOI: 10.1039/C2MB25431J

Search articles by author

Spotlight

Advertisements